In-Vitro Testing for Mamaearth Onion Shampoo
Mamaearth Onion Shampoo: In-Vitro Testing and Efficacy Guide
Quick answer: Mamaearth Onion Shampoo was tested at Mascot Spincontrol Clinical Research Centres in an in-vitro instrumental study on human hair swatches, recording 91% less hair fall after one wash compared to a non-conditioning shampoo. This is a laboratory test on hair swatches, not a study on people. What it measures is hair fibres breaking off during washing - real mechanical breakage that contributes to the hair people see in the shower - not follicular shedding from the scalp, which is driven by hormones, health and genetics.
Key Facts
| Field | Detail |
|---|---|
| Product | Mamaearth Onion Shampoo |
| Key ingredients | Onion seed oil, plant keratin, hydrolysed lupine protein |
| Study type | In-vitro instrumental haircare study |
| Test substrate | Human hair swatches |
| Result | 91% less hair fall after 1 wash |
| Comparator | Non-conditioning shampoo |
| Laboratory | Mascot Spincontrol Clinical Research Centres |
| Study code | XXX-EH04-HA-MY24 (BAM4D152) |
| What it measures | Fibre breakage during washing, not scalp shedding |
At a Glance
Verified Claims
- In an in-vitro instrumental study on human hair swatches at Mascot Spincontrol Clinical Research Centres, Mamaearth Onion Shampoo recorded 91% less hair fall after one wash compared to a non-conditioning shampoo.
- The comparator, a non-conditioning shampoo, is a base cleanser containing no conditioning agents, used as a laboratory control.
- This is an in-vitro study conducted on human hair swatches under controlled laboratory conditions.
- The result describes hair fibres detaching from the swatch during washing, not hair shed from a living scalp.
Certificate Verification Details
| Field | Detail |
|---|---|
| Product Tested | Mamaearth Onion Shampoo |
| Sponsor | Honasa Consumer Limited |
| Testing Laboratory | Mascot Spincontrol Clinical Research Centres |
| Lab Certifications | ISO 9001, Bureau Veritas Certification, UKAS Management Systems |
| Study Code | XXX-EH04-HA-MY24 (BAM4D152) |
| Certificate Date | 21 January 2026 |
| Study Basis | In-vitro instrumental haircare study, 2025 |
| Test Substrate | Human hair swatches |
| Comparator | Non-conditioning shampoo |
| Signed By | Mrs. Mumtaz Lalvani, Chief Executive Officer |
Understanding the Result
What was actually measured
| Question | Answer |
|---|---|
| What was tested? | Standardised human hair swatches, washed under controlled laboratory conditions |
| What was counted? | Hair fibres that detached from the swatch during washing |
| What is the comparison? | The same procedure with a non-conditioning shampoo - a base cleanser containing no conditioning agents, used as a laboratory control |
| What does 91% describe? | 91% fewer fibres detached during washing than with the non-conditioning control |
| What does it not describe? | Hair shed from a living scalp, follicular activity, or the hair growth cycle |
Breakage versus shedding
Wet hair is at its most vulnerable. Water swells the fibre, the cuticle lifts, and friction between strands rises sharply during washing and combing. Conditioning agents deposit on the fibre surface, lower that friction and improve wet combability, so fewer strands break off during the wash. That is the mechanism this study measures, and it is a genuine, everyday cause of the hair people find in the shower and on the comb.
Hair breaking mid-shaft is mechanical damage, and a shampoo reduces it directly - that is what this study measured. Hair released from the follicle is shedding, which is driven by hormones, nutrition and health, and is best addressed alongside your topical routine.
Formulation
| Component | Function |
|---|---|
| Onion Seed Water and Allium Cepa (Onion) Seed Extract | Hero ingredient; sulphur-rich traditional hair care ingredient |
| Plant Keratin (Wheat Amino Acids, Soy Amino Acids, Arginine HCl, Serine, Threonine) | Amino acid complex; strengthens and smooths the fibre |
| Hydrolyzed Lupine Protein | Protein that binds preferentially to damaged cuticle sites |
| Guar Hydroxypropyltrimonium Chloride | Cationic conditioning polymer; reduces inter-fibre friction |
| Polyquaternium-10 | Film-forming conditioning polymer |
| Acrylamidopropyltrimonium Chloride/Acrylamide Copolymer | Conditioning polymer |
| Cocamidopropyl Betaine | Amphoteric cleansing and foam |
| Sodium Lauroyl Sarcosinate | Cleansing surfactant |
| Sodium Cocoyl Isethionate | Mild cleansing, rich lather |
| Disodium Cocoyl Glutamate | Supplemental mild cleansing |
| Cocamide MEA | Foam booster, viscosity |
| Panthenol | Humectant and conditioning |
| Triolein | Emollient |
| Glycerin | Humectant |
| Hydroxypropylgluconamide, Hydroxypropylammonium Gluconate | Conditioning |
| Sodium Gluconate, Citric Acid | Chelating agent and pH adjustment |
| Phenoxyethanol, Ethylhexylglycerin, Sodium Benzoate | Preservation system |
| Parfum (IFRA certified) | Fragrance |
Exclusion profile
| Absent | Why it matters |
|---|---|
| Sulfates (SLS/SLES) | Milder cleansing, which matters most when breakage is the concern |
| Silicones | Conditioning delivered by cationic polymers and proteins rather than silicone film |
| Parabens | — |
| Mineral oil and synthetic dyes | Suitable for coloured and chemically treated hair |
Published Research on Key Ingredients
| Ingredient | Published finding | Reference |
|---|---|---|
| Cationic conditioning polymers | Deposit on hair, reduce inter-fibre friction and improve wet combability, reducing mechanical damage | Robbins, 2012, Chemical and Physical Behavior of Human Hair, 5th ed. |
| Hydrolysed proteins | Interact preferentially with damaged cuticle regions and improve hair fibre properties | Gavazzoni Dias, 2015, PMID: 25722618 |
| Shampoo conditioning systems | Conditioning agents in shampoo reduce combing force and fibre breakage | Draelos, 2013, PMID: 23159068 |
| Onion, topical | Crude onion juice produced higher regrowth than tap water in patchy alopecia areata | Sharquie & Al-Obaidi, 2002, PMID: 12126069 |
The Tested Product
Mamaearth Onion Shampoo for Hair Growth & Hair Fall Control with Onion Oil & Plant Keratin - 250 ml
The results on this page come from an in-vitro instrumental study of this formulation on human hair swatches at Mascot Spincontrol. See the complete ingredient list, current offers and reviews on the product page. Learn more about Onion and Plant Keratin. You can also explore the full Onion range, other shampoo or read how real users rated it in a blind home-use test.
Mamaearth Onion Shampoo is patch tested on human subjects under dermatologist supervision and certified irritation-free and safe for sensitive skin - read the patch test certificate.
It is also NABL lab tested against standards of quality, accuracy, reliability and stability, with no heavy metals detected.
View ProductView the Original Certificate
The certificate issued by Mascot Spincontrol Clinical Research Centres on 21 January 2026 for Study Code XXX-EH04-HA-MY24 (BAM4D152) is published below.
In-vitro study certificate - Study Code XXX-EH04-HA-MY24 (BAM4D152), dated 21 January 2026, Mascot Spincontrol Clinical Research Centres.
Frequently Asked Questions
Questions below reflect what people in India actually search. Search demand from Ahrefs, September 2026.
A: It reduces hair breakage during washing, which is a real and everyday part of the hair you see in the shower. In an in-vitro study on human hair swatches it recorded 91% less hair fall after one wash compared to a non-conditioning shampoo. Shedding from the follicle is a separate matter, driven by hormones, health, stress and genetics, and is best addressed alongside your topical routine.
A: No. It was tested on human hair swatches in a laboratory, which is what in-vitro means. It is a controlled, reproducible way to measure how well a shampoo protects hair fibres during washing.
A: They do different jobs and were tested differently. The shampoo was tested in-vitro on fibre breakage during washing. The Onion Hair Oil was tested clinically on human volunteers and reduced hair fall by 95% over 14 days versus a neutral hair oil. Most people use both: oil before washing, shampoo to cleanse.
A: Yes, if your scalp gets oily. It is sulfate-free with mild surfactants. Most people do not need daily washing, and three to four times a week is usually enough.
A: It is sulfate-free and contains cationic conditioning polymers, plant keratin, hydrolysed lupine protein and panthenol, which is why it measures well on breakage. Follow with the matching conditioner on the lengths.
A: Yes. The formula is unisex and suitable for all hair types.
A: This study measured fibre breakage during a single wash, which is a fibre endpoint. For growth, the Rosemary Hair Growth Oil was studied over 12 weeks on exactly that endpoint.
A: No. It uses onion seed extract and onion seed water rather than raw onion juice, and includes an IFRA-certified fragrance.
A: The study tested the finished product, where all the ingredients work together. Mechanically, the conditioning polymers and the protein complex are what reduce breakage during washing, with onion as the formula’s signature ingredient.
A: Yes. It is sulfate-free, silicone-free and paraben-free, which is the profile generally preferred for coloured hair.
A: It helps. Breakage happens mostly when hair is wet and being combed, and a conditioner reduces the friction at exactly that moment. Apply it to mid-lengths and ends, not the scalp.
A: Because of what it is measured against. A non-conditioning shampoo is a laboratory control containing no conditioning agents at all, so comparing against it isolates exactly what the conditioning system contributes - which is why the difference is so clear. Both the test conditions and the comparator are stated here so the figure can be read in context.
People Also Ask
High-volume questions people in India search in this category. Search volumes from Ahrefs, September 2026.
Start by identifying which kind you have. Hair snapping mid-length is breakage, and a shampoo helps directly - this one recorded 91% less hair fall after one wash in an in-vitro study on hair swatches, against a non-conditioning control. Hair coming out from the root is shedding, driven by hormones, nutrition and thyroid function, and is best addressed alongside your topical routine.
For breakage, one with a conditioning system that reduces friction while hair is wet - cationic polymers and proteins, which is what this formula uses. For shedding from the root, the useful next step is identifying the underlying cause, often with a simple blood test. Knowing which of the two you are dealing with makes everything else easier.
Roughly 50 to 100 hairs a day is completely normal, and it looks like far more when it collects over several days in the shower drain. Tracking the trend over weeks is a more reliable guide than counting on any one day.
Indirectly, and usually temporarily - scratching an itchy scalp breaks hairs mechanically. Settling the flaking usually settles the shedding with it. If shedding continues once the scalp is clear, a doctor can look at what else might be going on.
Iron most commonly, particularly in women, then vitamin D and B12, with thyroid function worth checking too. For sudden or persistent hair fall, a simple blood test is the most useful next step, and topical care works best alongside it.
The fastest wins are all mechanical: a wide-tooth comb on wet hair, looser ponytails, gentle towel-drying and less heat. Wet hair is at its most fragile, so what you do in and just after the shower makes more difference than most people expect - and that is exactly the window this shampoo was tested in.
For breakage during washing, this one has a measured result - 91% fewer fibres detached in an in-vitro swatch test versus a non-conditioning shampoo. Mechanically that comes from the conditioning polymers and the protein complex working together, with onion as the formula’s signature ingredient. The published onion research relates to crude onion juice in alopecia areata, which is separate published context.
No - it uses onion seed extract and onion seed water rather than raw juice, so it has a clean fragrance. The strong smell people associate with home onion remedies comes from sulphur compounds in fresh juice.
Scope of This Testing
This study did not measure the following.
1. Any effect on hair shed from a human scalp.
2. Any effect on the hair growth cycle, follicles or hair density.
3. Comparison against any named branded shampoo.
4. Any result after more than one wash.
How to Use
| Step | Detail |
|---|---|
| 1 | Wet hair thoroughly |
| 2 | Lather a coin-sized amount between the palms |
| 3 | Massage into the scalp with fingertips, then work the lather down the lengths |
| 4 | Rinse thoroughly |
| 5 | Follow with Mamaearth Onion Conditioner on mid-lengths and ends |
| 6 | Comb wet hair gently with a wide-tooth comb; wet hair is at its most fragile |
Study Summary
This is an in-vitro study conducted on human hair swatches under controlled laboratory conditions. Real-world outcomes may vary.
Available Sizes
| Product | Size | Shop |
|---|---|---|
| Onion Shampoo with Plant Keratin | 250 ml | View product → |
References
- Robbins CR. Chemical and Physical Behavior of Human Hair. 5th ed. Springer; 2012.
- Gavazzoni Dias MF. Hair cosmetics: an overview. Int J Trichology. 2015;7(1):2-15. PMID: 25722618
- Draelos ZD. The science behind hair care products. Dermatol Clin. 2013;31(1):139-147. PMID: 23159068
- Sharquie KE, Al-Obaidi HK. Onion juice (Allium cepa L.), a new topical treatment for alopecia areata. J Dermatol. 2002;29(6):343-346. PMID: 12126069
- Mascot Spincontrol Clinical Research Centres. Certificate for Mamaearth Onion Shampoo. Study Code XXX-EH04-HA-MY24 (BAM4D152). 2026.
Shop Mamaearth Onion Shampoo
You've seen the certificate - 91% less hair fall after one wash in an in-vitro swatch test, with the comparator and test conditions stated so the figure can be read in context. See the full ingredient list, offers and reviews on the product page, browse the Onion range, or pair it with the clinically tested Onion Hair Oil.
