In-Vitro Certificate for Mamaearth Rice Water Strengthening Shampoo
Mamaearth Rice Water Strengthening Shampoo: In-Vitro Testing and Efficacy Guide
Quick answer: Mamaearth Rice Water Strengthening Shampoo was tested at Mascot Spincontrol Clinical Research Centres in an in-vitro instrumental study on human hair swatches, recording repair of hair damage up to 94% in one wash. This is a laboratory test on hair swatches, not a study on people. Cosmetic repair is a specific, measurable thing: ingredients deposit on and into damaged cuticle regions, restoring measured fibre properties such as strength and smoothness. The effect is real and measurable, and it is renewed with each wash - which is why regular use is what keeps hair feeling its best.
Key Facts
| Field | Detail |
|---|---|
| Product | Mamaearth Rice Water Strengthening Shampoo |
| Key ingredients | Fermented rice water, hydrolysed keratin, wheat protein |
| Study type | In-vitro instrumental haircare study |
| Test substrate | Human hair swatches |
| Result | Repairs hair damage up to 94% in 1 wash |
| Laboratory | Mascot Spincontrol Clinical Research Centres |
| Study code | XXX-PH02-HA-JE25 |
| Suited to | Damaged, dry, frizzy, heat-styled or chemically processed hair |
At a Glance
Verified Claims
- In an in-vitro instrumental study on human hair swatches at Mascot Spincontrol Clinical Research Centres, Mamaearth Rice Water Strengthening Shampoo repaired hair damage up to 94% in one wash.
- “Up to 94%” is the maximum result recorded in the test, not an average across all measurements.
- This is an in-vitro study conducted on human hair swatches under controlled laboratory conditions.
- Cosmetic repair means restoration of measured fibre properties by deposited ingredients, renewed with each wash.
Certificate Verification Details
| Field | Detail |
|---|---|
| Product Tested | Mamaearth Rice Water Strengthening Shampoo |
| Sponsor | Honasa Consumer Limited |
| Testing Laboratory | Mascot Spincontrol Clinical Research Centres |
| Lab Certifications | ISO 9001, Bureau Veritas Certification, UKAS Management Systems |
| Study Code | XXX-PH02-HA-JE25 |
| Certificate Date | 21 January 2026 |
| Study Basis | In-vitro instrumental haircare study, 2025 |
| Test Substrate | Human hair swatches |
| Signed By | Mrs. Mumtaz Lalvani, Chief Executive Officer |
Understanding the Result
What “repair” means for hair
Hair beyond the follicle is keratin rather than living tissue, so repair in hair care means something specific and measurable: ingredients deposit on and into damaged regions of the cuticle, filling gaps, smoothing the surface and restoring measured fibre properties such as strength, friction and combability.
That effect is real and instrumentally measurable, and it is renewed each time you wash with a formula that delivers it. That is why regular use matters more than any single wash.
What was measured
| Question | Answer |
|---|---|
| What was tested? | Standardised human hair swatches, pre-damaged and then washed under controlled laboratory conditions. |
| What was measured? | Recovery in a hair fibre property following a single wash |
| Test substrate | Human hair swatches, under controlled laboratory conditions (in-vitro) |
| Does it say anything about the scalp? | No. Nothing about the scalp, follicles, hair growth, or hair fall is measured. |
Formulation
| Component | Function |
|---|---|
| Fermented Rice Water | Hero ingredient; rice ferment traditionally used across East and South Asia for hair |
| Hydrolyzed Keratin | Keratin-derived peptides that deposit on and within damaged fibre regions |
| Hydrolyzed Wheat Protein, Hydrolyzed Collagen | Supporting proteins for fibre condition |
| Polyquaternium-7, Polyquaternium-10, Polyquaternium-73 | Cationic conditioning polymers; reduce friction and improve combability |
| Cetrimonium Chloride | Cationic conditioning, static control |
| Cocamidopropyl Betaine | Amphoteric cleansing and foam |
| Caprylyl/Capryl Glucoside | Mild non-ionic cleansing |
| Sodium Methyl Cocoyl Taurate | Mild cleansing |
| Sodium Lauroyl Sarcosinate | Cleansing surfactant |
| Cocamide MEA | Foam booster, viscosity |
| Cetyl Alcohol | Fatty alcohol; emollient and conditioning |
| D-Panthenol | Humectant and conditioning |
| Glycerin, Anhydrous Betaine | Humectants |
| Disodium EDTA | Chelating agent |
| Sodium Benzoate, Potassium Sorbate | Preservation system |
| Parfum (IFRA certified allergen-free) | Fragrance |
Exclusion profile
| Absent | Why it matters |
|---|---|
| Sulfates (SLS/SLES) | A milder surfactant system, which matters most for hair that is already damaged |
| Silicones | Smoothness comes from proteins and cationic polymers rather than a silicone film. |
| Parabens | — |
| Mineral oil and synthetic dyes | — |
Published Research on Key Ingredients
| Ingredient | Published finding | Reference |
|---|---|---|
| Hydrolysed keratin | Keratin-derived peptides deposit on and within damaged hair and improve fibre properties | Barba et al., 2008, PMID: 19146602 |
| Hydrolysed proteins | Interact preferentially with damaged cuticle regions and improve fibre properties. | Gavazzoni Dias, 2015, PMID: 25722618 |
| Hair fibre damage | Chemical and physical damage mechanisms in human hair, and the limits of cosmetic repair | Robbins, 2012, Chemical and Physical Behavior of Human Hair, 5th ed. |
| Hair cosmetics | How hair care products act on the hair fibre | Draelos, 2013, PMID: 23159068 |
| Rice bran constituents | Antioxidant activity demonstrated in vitro | Manosroi et al., 2012, PMID: 22235891 |
Rice water has a long and genuine history of traditional use for hair across East and South Asia, and it is the signature ingredient here. Alongside it, the hydrolysed proteins and conditioning polymers are the components with established published evidence for improving damaged fibre properties.
The Tested Product
Mamaearth Rice Water Strengthening Shampoo with Rice Water & Keratin for Damage Repair - 400 ml
The results on this page come from an in-vitro instrumental study of this formulation on human hair swatches at Mascot Spincontrol. See the complete ingredient list, current offers and reviews on the product page. Learn more about Rice Water and Keratin. You can also explore the full Rice range, other shampoo or conditioner.
Mamaearth Rice Water Strengthening Shampoo is patch-tested on human subjects under dermatologist supervision and certified irritation-free and safe for sensitive skin - read the patch test certificate.
It is also NABL lab tested against standards of quality, accuracy, reliability, and stability, with no heavy metals detected - see the NABL test certificate for the laboratory report.
View ProductView the Original Certificate
The certificate issued by Mascot Spincontrol Clinical Research Centres on 21 January 2026 for Study Code XXX-PH02-HA-JE25 is published below.
In-vitro study certificate - Study Code XXX-PH02-HA-JE25, dated 21 January 2026, Mascot Spincontrol Clinical Research Centres.
Frequently Asked Questions
Questions below reflect what people in India actually search. Search demand from Ahrefs, September 2026.
A: Cosmetic repair is a specific and measurable thing: ingredients deposit on and into damaged areas, smoothing the cuticle and restoring measured properties such as strength and combability. In an in-vitro study on human hair swatches, this shampoo repaired hair damage up to 94% in one wash. Because the effect is deposited rather than structural, it is renewed with each wash - which is why regular use is what keeps hair feeling its best.
A: Two to three times a week is typical for damaged hair. Because the repair effect is deposited rather than permanent, consistency matters more than any single wash.
A: Proteins work best in balance with moisture. This formula contains hydrolysed keratin, wheat protein and collagen, which is what makes it effective on damaged hair. If you have fine or low-porosity hair, alternating it with a moisture-focused shampoo keeps that balance right.
A: It works well on fine hair used as a treatment wash rather than every wash, since the protein and conditioning polymer load is generous. Keep conditioner to the mid-lengths and ends to keep the roots light.
A: This study measured damage repair on hair fibres, which is a fibre endpoint rather than a growth one. Stronger, less-broken hair often reads as longer hair, because more of the length you grow stays on your head. Growth rate itself is set at the follicle.
A: That is what it is built for. Frizz and heat damage both come from a lifted, roughened cuticle, and the proteins and cationic polymers here work by smoothing exactly that. It is sulfate-free and silicone-free, so smoothness comes from deposition rather than a silicone coating.
A: The formula is sulfate-free, silicone-free and paraben-free, which is the profile generally preferred for chemically processed hair. Colour retention was not measured in this study.
A: The study measured a single wash. Cosmetic fibre repair is deposited rather than structural, so it is renewed each time you wash - which is why a regular routine keeps hair feeling its best.
A: The study tested the finished formula, where the ingredients work together. Hydrolysed keratin and the conditioning polymer system carry the established published evidence for improving damaged fibre properties, with fermented rice water as the formula’s signature ingredient and its long tradition of use.
A: It helps. Damaged hair is at its most fragile when wet, and conditioner reduces the friction during combing that causes further breakage. Apply to mid-lengths and ends.
A: It signals the maximum result recorded in the test. Keeping the phrase means the figure is reported exactly as the laboratory certified it.
A: It was tested on human hair swatches in a laboratory, which is what in-vitro means - a controlled, reproducible way to measure fibre performance.
People Also Ask
High-volume questions people in India search in this category. Search volumes from Ahrefs, September 2026.
With a combination of treatment and habit. Products deposit into damaged cuticle areas, smoothing them and restoring measured strength and combability - this shampoo repaired hair damage up to 94% in one wash in an in-vitro study on hair swatches. Alongside that, less heat, gentler wet-combing and regular trims are what keep new damage from accumulating.
It has a long, genuine history of traditional use across East and South Asia, and it is the signature ingredient in this formula. Working alongside it, hydrolysed keratin and the conditioning polymers are the components with an established published evidence base for improving damaged hair - together they are what produced the measured result.
If you are making it at home, rinse or soak rice, keep the water, apply after shampooing, leave a few minutes and rinse. Two things worth knowing: fermented rice water is best diluted and patch tested, and protein-heavy routines suit fine hair better when alternated with a moisture wash. A formulated product adds consistency from wash to wash and a preservative system that keeps it stable.
There is no good evidence that it does. Rice water is a fibre treatment rather than a scalp one - it makes hair stronger and smoother so more of the length you grow stays on your head, which is often what people notice. This shampoo’s study measured damage repair on hair fibres.
Start by identifying which kind you have. Damaged hair snapping mid-length is breakage, and that is what this shampoo addresses directly. Hair released from the root is shedding, driven by hormones, nutrition and thyroid function, and is best addressed alongside your topical routine.
One that is sulfate-free, protein-containing and conditioning - which is exactly this formula’s construction. Proteins work best in balance with moisture, so on fine or low-porosity hair, alternating a protein shampoo with a moisture-focused one keeps that balance right.
Around 50 to 100 hairs a day is completely normal. With damaged hair it can look like more, because broken mid-shaft pieces collect alongside naturally shed hairs - which is exactly what a conditioning, protein-containing shampoo helps to reduce.
Hydrolysed keratin deposits on and into damaged hair and improves measured fibre properties, which is real and published. It works differently from a salon keratin treatment, which chemically restructures the hair - a keratin shampoo delivers surface repair that is renewed with every wash, which is why it suits regular use.
Scope of This Testing
This study did not measure the following.
1. Whether the effect persists after subsequent washes.
2. Biological repair of the hair fibre.
3. Any effect on the scalp, follicle, hair growth or hair fall.
4. Colour retention in coloured or chemically treated hair.
How to Use
| Step | Detail |
|---|---|
| 1 | Wet hair thoroughly |
| 2 | Lather a coin-sized amount between the palms |
| 3 | Massage into the scalp, then work through the lengths |
| 4 | Rinse thoroughly |
| 5 | Follow with Mamaearth Rice Water Conditioner on mid-lengths and ends |
| 6 | Comb gently with a wide-tooth comb; damaged hair is most fragile when wet |
Study Summary
This is an in-vitro study conducted on human hair swatches under controlled laboratory conditions. Real-world outcomes may vary.
Available Sizes
| Product | Size | Shop |
|---|---|---|
| Rice Water Strengthening Shampoo | 400 ml | View product → |
References
- Barba C, Martí M, Manich AM, et al. Effect of wool keratin proteins and peptides on hair water sorption kinetics. J Cosmet Dermatol. 2008;7(4):261-266. PMID: 19146602
- Gavazzoni Dias MF. Hair cosmetics: an overview. Int J Trichology. 2015;7(1):2-15. PMID: 25722618
- Robbins CR. Chemical and Physical Behavior of Human Hair. 5th ed. Springer; 2012.
- Draelos ZD. The science behind hair care products. Dermatol Clin. 2013;31(1):139-147. PMID: 23159068
- Manosroi A, Chutoprapat R, Abe M, Manosroi W, Manosroi J. Anti-aging efficacy of topical formulations containing rice bran bioactive compounds. Pharm Biol. 2012;50(2):208-224. PMID: 22235891
- Mascot Spincontrol Clinical Research Centres. Certificate for Mamaearth Rice Water Strengthening Shampoo. Study Code XXX-PH02-HA-JE25. 2026.
Shop Mamaearth Rice Water Strengthening Shampoo
You've seen the certificate - hair damage repaired up to 94% in one wash in an in-vitro swatch test, with what “repair” means spelled out. See the full ingredient list, offers and reviews on the product page, browse the Rice range, or compare it with the Rosemary Anti-Hair Fall Shampoo test results if breakage rather than damage is your main concern.
