Clinical Testing Report for Mamaearth Lemon Anti-Dandruff Shampoo and Conditioner
Mamaearth Lemon Anti-Dandruff Shampoo & Conditioner: Clinical Testing and Efficacy Guide
Quick answer: Mamaearth Lemon Anti-Dandruff Shampoo and Conditioner were tested by CCFT Laboratories in an anti-dandruff efficacy evaluation on human volunteers under controlled conditions. The study substantiated up to 100% dandruff reduction in one wash and 3X softer, smoother hair in one wash. The protocol is registered with the Clinical Trials Registry - India under CTRI/2025/08/092241 and is publicly searchable at ctri.nic.in. The anti-dandruff active in the formula is zinc pyrithione, an established antifungal that acts on Malassezia, the yeast most associated with dandruff.
Key Facts
| Field | Detail |
|---|---|
| Products | Mamaearth Lemon Anti-Dandruff Shampoo and Conditioner |
| Anti-dandruff active | Zinc pyrithione |
| Supporting ingredients | Lemon fruit extract, ginger extract |
| Study type | Anti-dandruff efficacy evaluation on human volunteers (in vivo) |
| Laboratory | CCFT Laboratories Pvt. Ltd., Meerut |
| Registration | CTRI/2025/08/092241 |
| Verifiable at | ctri. nic. in |
| Result | Up to 100% dandruff reduction in 1 wash |
| Recommended frequency | 3 times a week |
At a Glance
On “up to 100%”: this is the maximum result observed in the study, not the panel average. How much dandruff clears in a single wash depends on your starting severity and on what is driving it.
Verified Claims
- In an anti-dandruff efficacy evaluation conducted by CCFT Laboratories on human volunteers under controlled conditions, Mamaearth Lemon Anti-Dandruff Shampoo and Conditioner substantiated up to 100% dandruff reduction in one wash.
- In the same evaluation, the products delivered 3X softer, smoother hair in one wash.
- The study protocol is registered with the Clinical Trials Registry - India under CTRI/2025/08/092241 and is publicly searchable at ctri.nic.in.
- The formula’s anti-dandruff active is zinc pyrithione, a well-established antifungal agent used against Malassezia.
Certificate Verification Details
| Field | Detail |
|---|---|
| Products Tested | Mamaearth Lemon Anti-Dandruff Shampoo and Mamaearth Lemon Anti-Dandruff Conditioner |
| Sponsor | Honasa Consumer Limited |
| Testing Laboratory | CCFT Laboratories Pvt. Ltd., Meerut |
| Lab Designation | FIST Centre by Dept. of Science & Technology, Govt. of India |
| Lab Certifications | ISO 9001:2015, ISO 45001:2015, OECD guidelines GLP compliant |
| CTRI Registration | CTRI/2025/08/092241 |
| Study Type | Anti-dandruff efficacy evaluation on human volunteers |
| Conditions | Controlled |
| Signed By | Puneet Mittal, Director R&D; Nidhi Dixit, Chief Scientific Officer |
How This Formula Works
| Element | Role |
|---|---|
| Zinc pyrithione | The anti-dandruff active. An established antifungal with decades of published clinical evidence against Malassezia, the yeast most closely associated with dandruff. This is the ingredient responsible for the dandruff result. |
| Lemon (Citrus Limon fruit extract) | Astringent and antioxidant; supports cleansing of flakes and excess scalp oil |
| Ginger (Zingiber Officinale extract) | Soothing ingredient for scalp comfort |
| Conditioning system | Delivers the softness result and offsets the roughness common to anti-dandruff formulas |
Why softness was measured alongside dandruff: people generally want a clear scalp without trading away hair feel, so the study measured both on the same wash - dandruff control and softness together, rather than one at the expense of the other.
Formulation
Shampoo
| Component | Function |
|---|---|
| Zinc Pyrithione | Anti-dandruff active; antifungal |
| Citrus Limon (Lemon) Fruit Extract | Hero ingredient; astringent, antioxidant |
| Zingiber Officinale (Ginger) Extract | Soothing, scalp comfort |
| Cocamidopropyl Betaine | Amphoteric cleansing and foam |
| Lauroyl/Myristoyl Methyl Glucamide | Mild non-ionic cleansing surfactant |
| Cetrimonium Chloride | Cationic conditioning, static control |
| Guar Hydroxypropyltrimonium Chloride | Conditioning, improves combability |
| Panthenol | Humectant and conditioning |
| Disodium EDTA | Chelating agent |
| Preservation system | Product stability |
| Cooling agent, Parfum (IFRA certified allergen-free) | Sensory |
Conditioner
| Component | Function |
|---|---|
| Cocos Nucifera (Coconut) Oil | Conditioning, frizz control, smoothing |
| Citrus Limon (Lemon) Fruit Extract | Hero ingredient |
| Cationic conditioning agents | Softness, combability, static control |
| Fatty alcohols and emollients | Slip and smoothness |
| Preservation system | Product stability |
Exclusion profile
| Absent | Why it matters |
|---|---|
| Sulfates (SLS/SLES) | A milder cleansing system on a scalp that is already flaking and irritated |
| Parabens | — |
| Silicones | Conditioning delivered by cationic agents rather than a silicone film |
| Mineral oil and synthetic dyes | — |
Published Research on Key Ingredients
| Ingredient | Published finding | Reference |
|---|---|---|
| Zinc pyrithione | Established antifungal efficacy against Malassezia; the mainstay over-the-counter anti-dandruff active. | Schwartz et al., 2005, PMID: 16382662 |
| Zinc pyrithione | Antifungal mechanism involves zinc-mediated disruption of iron-sulfur proteins in fungal metabolism. | Reeder et al., 2011, PMID: 21614016 |
| Malassezia | Causal role established in dandruff and seborrhoeic dermatitis | Gaitanis et al., 2012, PMID: 22232373 |
| Dandruff pathophysiology | Dandruff results from Malassezia, sebum, and individual susceptibility acting together | DeAngelis et al., 2005, PMID: 16382685 |
| Ginger | Anti-inflammatory activity attributed to gingerols and shogaols | Mashhadi et al., 2013, PMID: 23717767 |
Certificate Verification
| Field | Detail |
|---|---|
| Testing Laboratory | CCFT Laboratories Pvt. Ltd., Meerut |
| Registered Address | C-10/3, Jwala Nagar, T.P. Nagar, Meerut 250002, India |
| Lab Address | Centre for Cruelty-Free Testing, Meerut Institute of Engineering & Technology, NH-58, Delhi-Roorkee Highway, Baghpat Bypass Road Crossing, Meerut 250005 |
| Lab Contact | +91-8937045757 (India), +1-510-500-5624 (USA), +44-741-834-4803 (UK) |
| Website | www.centreforcrueltyfreetesting.com |
| CTRI Verification | CTRI/2025/08/092241, verifiable at ctri. nic. in |
| Signed By | Puneet Mittal, Director R&D; Nidhi Dixit, Chief Scientific Officer |
The Tested Product
Mamaearth Lemon Anti-Dandruff Shampoo with Lemon & Ginger for up to 100% Dandruff & Itch-Free Scalp - 250 ml
The results on this page come from a CTRI-registered efficacy evaluation of this shampoo and its matching conditioner on human volunteers at CCFT Laboratories, Meerut. See the complete ingredient list, current offers, and reviews on the product page. Learn more about Zinc Pyrithione, Lemon and Ginger. You can also explore the full anti-dandruff range, other shampoos or conditioners.
Mamaearth Lemon Anti-Dandruff Shampoo is patch-tested on human subjects under dermatologist supervision and certified irritation-free and safe for sensitive skin - read the patch test certificate.
It is also NABL lab tested against standards of quality, accuracy, reliability, and stability, with no heavy metals detected.
View ProductView the Original Certificate
The claim sheet issued by CCFT Laboratories Pvt. Ltd. for the CTRI-registered anti-dandruff efficacy evaluation (CTRI/2025/08/092241) is published below.
Anti-dandruff efficacy evaluation claim sheet - CCFT Laboratories Pvt. Ltd., Meerut, CTRI/2025/08/092241, signed by Puneet Mittal, Director R&D, and Nidhi Dixit, Chief Scientific Officer.
Open Certificate (PDF) →Frequently Asked Questions
Questions below reflect what people in India actually search. Search demand from Ahrefs, September 2026.
A: In a CTRI-registered study conducted by CCFT Laboratories on human volunteers under controlled conditions, the shampoo and conditioner substantiated up to 100% dandruff reduction in one wash. The active ingredient doing that work is zinc pyrithione, which has decades of published clinical evidence behind it. The study registration, CTRI/2025/08/092241, is searchable at ctri.nic.in.
A: Three times a week is the standard guidance for an anti-dandruff shampoo. Dandruff is a recurring condition, so consistency matters more than any single wash. Once your scalp is clear, many people drop to once or twice a week to maintain.
A: Dandruff is driven by Malassezia yeast, sebum, and individual susceptibility, so it responds best to an ongoing routine rather than a one-off course. Keeping the shampoo in rotation is what keeps a scalp clear.
A: That is the standard complaint about anti-dandruff shampoos, and it is why softness was measured in the same study - 3X softer and smoother hair in one wash. The formula is sulfate-free and includes cationic conditioning agents and panthenol. Using the matching conditioner is how the products were tested.
A: No. Zinc pyrithione is the anti-dandruff active. Lemon and ginger contribute to cleansing, scalp comfort, and the sensory experience. Being clear about this is the point: the 100% figure is credible precisely because a proven antifungal is doing the work.
A: You can, though most people find three times a week is all they need. The active works through consistency rather than frequency, so a steady routine gets you further than daily washing.
A: Both target Malassezia, with different actives. Ketoconazole shampoos are typically pharmacy products used for more stubborn dandruff and for seborrhoeic dermatitis. This is a cosmetic anti-dandruff shampoo using zinc pyrithione, designed for regular use. If dandruff persists despite a consistent routine, a doctor can advise on next steps.
A: Dandruff and itch usually travel together, since both stem from the same scalp irritation, so settling one tends to settle the other. The formula includes ginger extract and a cooling agent for scalp comfort, and the study substantiated dandruff reduction as its certified endpoint.
A: The shampoo and conditioner were tested together as a regimen, and the 3X softness result reflects that pairing. Use the conditioner on mid-lengths and ends, keeping it off the scalp.
A: The formula is sulfate-free, silicone-free, and paraben-free, which is the profile generally preferred for coloured hair. Colour retention itself is measured in a separate kind of study.
A: This study covers cosmetic dandruff, not diagnosed conditions. Seborrheic dermatitis and scalp psoriasis need medical management, and anyone with either should follow their dermatologist’s advice.
A: Yes. Give it 2 to 3 minutes on the scalp before rinsing so the active has contact time. Rinsing immediately is one of the most common reasons an anti-dandruff shampoo underperforms.
A: Yes. CTRI/2025/08/092241 is a Clinical Trials Registry - India number, searchable at ctri.nic.in. The record exists independently of Mamaearth, which is why it is printed on the certificate and on this page.
People Also Ask
High-volume questions people in India search in this category. Search volumes from Ahrefs, September 2026.
With an active ingredient used consistently, not with a one-off wash. Zinc pyrithione, ketoconazole, and selenium sulphide are the ingredients with real evidence, and they work by controlling Malassezia, the scalp yeast behind most dandruff. This shampoo uses zinc pyrithione and, in a CTRI-registered study, substantiated up to 100% dandruff reduction in one wash. Use it three times a week, leave it on the scalp 2–3 minutes before rinsing, and keep going once it clears.
Dandruff is a condition you keep under control rather than clear once, because Malassezia yeast is a normal resident of nearly everyone’s scalp. What changes with treatment is whether it causes flaking - and that responds well to consistency. Regular use of an active shampoo keeps a scalp clear, which is why the routine matters as much as the product.
Three things together: Malassezia yeast feeding on scalp oils, how much sebum you produce, and how sensitive your particular scalp is to the by-products. That third factor is why two people with the same yeast levels can experience it very differently. Cold weather, stress, and washing less often all influence it, and regular washing with an active shampoo is what keeps it settled.
Indirectly, and usually temporarily. The follicle itself is unaffected, but scratching an itchy scalp breaks hairs mechanically, so hair fall and flaking often travel together. The good news is that settling the dandruff usually settles the shedding with it. If shedding continues once the scalp is clear, a doctor can look at what else might be going on.
The one whose active ingredient you can name. Zinc pyrithione, ketoconazole, and selenium sulphide all have published evidence; botanical-only shampoos generally do not. This shampoo uses zinc pyrithione, and its efficacy study is registered at CTRI/2025/08/092241, searchable at ctri.nic.in, which means you can check it independently of the brand.
Partly. Malassezia is a yeast, a type of fungus, and it lives on almost everyone’s scalp normally. Dandruff arises from how that yeast, your sebum, and your scalp’s sensitivity interact - which is exactly why an antifungal active such as zinc pyrithione is the ingredient to look for.
No - it is not passed between people. The yeast involved is already a normal resident on nearly everyone’s skin, so sharing a comb or a pillow makes no difference.
Visible flakes can clear substantially in a single wash with an effective active - this shampoo’s study substantiated up to 100% dandruff reduction in one wash, “up to” meaning the best result observed. To get the most from each wash, give it 2 to 3 minutes of contact time on the scalp before rinsing, and keep the routine going three times a week so the scalp stays clear.
Alongside an active shampoo, a few habits make a real difference: wash regularly rather than less often, keep heavy oiling light while the scalp is flaking since Malassezia feeds on lipids, and let the scalp settle rather than scratching. Home remedies such as lemon, curd, or apple cider vinegar are widely shared; if you use them, dilute well and patch test, and keep the active shampoo as the mainstay.
Scope of This Testing
This study did not measure the following.
1. The panel average - “up to 100%” is a maximum.
2. How long the effect lasts after a single wash.
3. Prevention of dandruff recurrence.
4. Efficacy in seborrhoeic dermatitis, scalp psoriasis, or any diagnosed condition.
5. Colour retention in coloured or chemically treated hair.
How to Use
| Step | Detail |
|---|---|
| 1 | Wet hair thoroughly |
| 2 | Apply a coin-sized amount and work into a lather |
| 3 | Focus on the scalp rather than the lengths |
| 4 | Leave on the scalp for 2–3 minutes so the active has contact time |
| 5 | Rinse thoroughly |
| 6 | Apply Lemon Anti-Dandruff Conditioner to mid-lengths and ends, then rinse |
| Frequency | 3 times a week |
Study Summary
Results reflect testing on the commercial formulation under controlled conditions. Individual results may vary.
Available Sizes & Combos
| Product | Size | Shop |
|---|---|---|
| Lemon Anti-Dandruff Shampoo | 250 ml | View product → |
| Lemon Dandruff Treatment Combo (Shampoo + Conditioner) | Combo | View product → |
References
- Schwartz JR, Rocchetta H, Asawanonda P, Luo F, Thomas JH. Does tachyphylaxis occur in long-term management of scalp seborrheic dermatitis with pyrithione zinc-based treatments? J Investig Dermatol Symp Proc. 2005;10(3):194-197. PMID: 16382662
- Reeder NL, Kaplan J, Xu J, et al. Zinc pyrithione inhibits yeast growth through copper influx and inactivation of iron-sulfur proteins. J Invest Dermatol. 2011;131(9):1801-1808. PMID: 21614016
- Gaitanis G, Magiatis P, Hantschke M, Bassukas ID, Velegraki A. The Malassezia genus in skin and systemic diseases. Clin Microbiol Rev. 2012;25(1):106-141. PMID: 22232373
- DeAngelis YM, Gemmer CM, Kaczvinsky JR, Kenneally DC, Schwartz JR, Dawson TL. Three etiologic facets of dandruff and seborrheic dermatitis. J Investig Dermatol Symp Proc. 2005;10(3):295-297. PMID: 16382685
- Mashhadi NS, Ghiasvand R, Askari G, Hariri M, Darvishi L, Mofid MR. Antioxidative and anti-inflammatory effects of ginger. Int J Prev Med. 2013;4(Suppl 1):S36-S42. PMID: 23717767
- CCFT Laboratories Pvt. Ltd. Claim Sheet, Anti-Dandruff Efficacy Evaluation. CTRI/2025/08/092241. 2025.
Shop Mamaearth Lemon Anti-Dandruff Shampoo
You've seen the certificate - up to 100% dandruff reduction and 3X softer hair in one wash, in a CTRI-registered study; you can verify it yourself at ctri.nic.in. See the full ingredient list, offers, and reviews on the product page, browse the anti-dandruff range, or read how Mamaearth Anti-Dandruff Shampoo performed in a blind consumer test.
